clusterin molecular weight

Biochim Biophys Acta. [, Kapron JT, Hilliard GM, Lakins JN, Tenniswood MP, West KA, Carr SA, Crabb JW: Identification and characterization of glycosylation sites in human serum clusterin. Nat Cell Biol. Elastin and clusterin were detected by fluorescence at an excitation wavelength of 275 nm and an emission wavelength of 307 nm using a FP 920 fluorescence detector (Jasco, Grossumstadt, Germany). Epub 2011 Apr 20. In clear cell renal cell carcinoma, CLU functions to regulate ERK 1/2 signaling and matrix metallopeptidase-9 expression to promote tumor cell migration, invasion, and metastasis. Interacts with APOA1, LRP2, CLUAP1 and PON1. [, Leskov KS, Klokov DY, Li J, Kinsella TJ, Boothman DA: Synthesis and functional analyses of nuclear clusterin, a cell death protein. [9] CLU expression in the kidney also plays a role in renal diseases, such as nephropathic cystinosis, which is a major cause of Fanconi syndrome. 1997 Oct;6(10):2120-33. 2005 Nov-Dec;4(6):2070-80. [, Liu T, Qian WJ, Gritsenko MA, Camp DG 2nd, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. There are no reviews for Clusterin/APOJ Antibody (NBP1-68308). Slightly acidic pH promotes interaction with misfolded proteins. [7] The encoded protein isoforms all localize to different subcellular compartments: one isoform localizes to the nucleus; a second isoform localizes to the cytoplasm; and the third is secreted from the cell. Interacts with the complement complex. Epub 2006 Dec 4. Biochem J. Mol Cancer Res. [, Kirszbaum L, Sharpe JA, Murphy B, d'Apice AJ, Classon B, Hudson P, Walker ID: Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa). [, Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. [, de Silva HV, Stuart WD, Park YB, Mao SJ, Gil CM, Wetterau JR, Busch SJ, Harmony JA: Purification and characterization of apolipoprotein J. J Biol Chem. 1992 Oct;112(4):557-61. BMC Genomics. J Mol Biol. 2010 Jan;8(1):119-30. doi: 10.1158/1541-7786.MCR-09-0277. Overexpression of clusterin appears to be a â¦ [, Ranney MK, Ahmed IS, Potts KR, Craven RJ: Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. [, Zhang H, Li XJ, Martin DB, Aebersold R: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. 2007 May 25;369(1):157-67. 2007 Aug;21(10):2312-22. Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Clusterin (cat. The first of these criteria was satisfied by a recent report that clusterin forms high-molecular-weight âsolubilizedâ complexes with heat- or reduction-stressed proteins, inhibiting their precipitation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody (ab69644) ab69644 at 1/50 dilution staining Apolipoprotein J in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide. Nature. Traffic. 1990 Jun 5;29(22):5380-9. Eur J Biochem. Humphreys D. et al. It is encoded by one single gene and cleaved posttranslationally into Î± (34-36 kDa) and Î² â¦ The chaperone protein then aids hepatitis C viral assembly by stabilizing its core and NS5A units. 2006 Apr;8(4):565-80. 2010 Feb 5;285(6):3532-9. doi: 10.1074/jbc.M109.079566. Western blot of HRP anti-Clusterin antibody (clone A15113A). CLU may promote tumorigenesis by facilitating BAX-KLU70 binding and, consequently, preventing BAX from localizing to the outer mitochondrial membrane to stimulate cell death. Biochemistry. 2007 Feb 6;46(5):1412-22. [, Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . SEC-purified HMW complexes were collected from fractions between 7â8 ml in the peak corresponding to the size exclusion limit of 4 107 Da. [9] The gene is highly conserved in species, and the protein is widely distributed in many tissues and organs, where it participates in a number of biological processes, including lipid transport, membrane recycling, cell adhesion, programmed cell death, and complement-mediated cell lysis. [, Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state. [, Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. [, Hochstrasser AC, James RW, Martin BM, Harrington M, Hochstrasser D, Pometta D, Merril CR: HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing. [7][8] The nuclear isoform encodes a 49 kDa protein, while the secretory isoform, which is the main gene transcript, encodes a 75â80 kDa protein after maturation (glycosylation, secretion, and dimerization). 2007 Oct 31;8:399. Studies revealed that inhibition of CLU resulted in increased effectiveness of chemotherapeutic agents to kill tumor cells. Forms high-molecular weight oligomers upon interaction with misfolded proteins. 1991 May-Jun;11(3):645-52. 2009 May;8(5):913-23. doi: 10.1074/mcp.M800504-MCP200. Mol Cell Proteomics. 2006 Feb;5(2):226-33. 1990 Aug 25;265(24):14292-7. After further adsorption with CLU, the particle size was 151.8 nm. 1994 May 1;221(3):917-25. 2004 Feb;4(2):454-65. 1 , C and D ). [, Choi NH, Mazda T, Tomita M: A serum protein SP40,40 modulates the formation of membrane attack complex of complement on erythrocytes. Protein Sci. The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . Epub 2013 Nov 22. [, Wyatt AR, Yerbury JJ, Berghofer P, Greguric I, Katsifis A, Dobson CM, Wilson MR: Clusterin facilitates in vivo clearance of extracellular misfolded proteins. Epub 2007 Apr 5. [, Andersen CL, Schepeler T, Thorsen K, Birkenkamp-Demtroder K, Mansilla F, Aaltonen LA, Laurberg S, Orntoft TF: Clusterin expression in normal mucosa and colorectal cancer. EMBO J. Forms high-molecular weight oligomers upon interaction with misfolded proteins. Clusterin-Î± Antibody (M-18) is replaced by a more specific monoclonal antibody, Clusterin-Î± (B-5) that gives a stronger signal & more consistent results. Introduction. Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). 2009 Feb;8(2):651-61. doi: 10.1021/pr8008012. Proteomics. Epub 2009 Jun 17. Cell Mol Life Sci. CLU promotes cell survival by a number of means, including inhibition of BAX on the mitochondrial membrane, activation of the phosphatidylinositol 3-kinase/protein kinase B pathway, modulation of extracellularular signal-regulated kinase (ERK) 1/2 signaling and matrix metallopeptidase-9 expression, promotion of angiogenesis, and mediation of the nuclear factor kappa B (NF-ÎºB) pathway. [7][8][9], Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and erythrocytes, hence its name. [7][8][9], The CLU gene contains nine exons and expresses three isoforms alternatively-spliced at the first exon. We are studying a form of apomixis called apospory that occurs in the genus Pennisetum , a taxon in the grass family. [8] In addition to the above diseases, CLU has been linked to other conditions resulting from oxidative damage, including aging, glomerulonephritis, atherosclerosis, and myocardial infarction. J Proteome Res. Epub 2007 Jul 4. [7], Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. N-glycosylation at six glycosylation sites is the final step in the generation of mCLU which, under nonreducing conditions, has a molecular weight of 75â80 kDa ( Figure 1A) [ 40 ]. Proc Natl Acad Sci U S A. The blot was incubated with â¦ Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Epub 2003 May 18. [, Santilli G, Aronow BJ, Sala A: Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity. [PubMed:8181474] Optimal dilutions/concentrations should be determined by the end user. FEBS Lett. Other cancers CLU has been implicated in include breast cancer, pancreatic cancer, hepatocellular carcinoma, and melanoma. Furthermore, in the absence of the leader sequence, the expressed clusterin had a molecular weight consistent with that of the predicted holoprotein (40 kDa), suggesting a compromised postâtranslational processing with diffuse distribution throughout the cytoplasm. molecular weight calibration kit (GE Healthcare). Not yet tested in other applications. [, Jia W, Lu Z, Fu Y, Wang HP, Wang LH, Chi H, Yuan ZF, Zheng ZB, Song LN, Han HH, Liang YM, Wang JL, Cai Y, Zhang YK, Deng YL, Ying WT, He SM, Qian XH: A strategy for precise and large scale identification of core fucosylated glycoproteins. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. p53 may also transcriptionally repress secretory CLU to further promote the proapoptotic cascade. 2003 Jun;21(6):660-6. [, Bunkenborg J, Pilch BJ, Podtelejnikov AV, Wisniewski JR: Screening for N-glycosylated proteins by liquid chromatography mass spectrometry. J Biol Chem. Biochim Biophys Acta. All buffers and samples were filtered (0.45 m) before use. Supplied as 150 µL purified antibody (0.2 mg/mL). Clusterin (CLU) is an extracellular chaperone that forms stable and soluble complexes w â¦ Epub 2005 Aug 21. [, Nizard P, Tetley S, Le Drean Y, Watrin T, Le Goff P, Wilson MR, Michel D: Stress-induced retrotranslocation of clusterin/ApoJ into the cytosol. J Biol Chem. 2005 Sep;7(9):909-15. Nat Genet. In epithelial ovarian cancer, CLU has been observed to promote angiogenesis and chemoresistance. [7] In particular, custirsen, an antisense oligonucleotide that blocks the CLU mRNA transcript, enhanced heat-shock protein 90 (HSP90) inhibitor activity by suppressing the heat-shock response in castrate-resistant prostate cancer, and is currently in phase III trials. Studies have shown that clusterin may be a useful diagnostic marker for ALCL, especially in ALK-1(-) cases. 2002 Oct 18;277(42):39532-40. [, Kirszbaum L, Bozas SE, Walker ID: SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. Clusterin (CLU) is a gene/protein highly conserved and expressed in several human tissues and fluids. 2002 Jun;269(11):2789-94. It is recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. [, Choi-Miura NH, Takahashi Y, Nakano Y, Tobe T, Tomita M: Identification of the disulfide bonds in human plasma protein SP-40,40 (apolipoprotein-J). [8][9], CLU has been shown to interact with Ku70. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. [, Ghiso J, Matsubara E, Koudinov A, Choi-Miura NH, Tomita M, Wisniewski T, Frangione B: The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseasâ¦ Biochemistry. The integrity of the complexes was checked peri-odically by reanalysis using the same column. J Biol Chem. Unlike most other chaperone proteins, which aid intracellular proteins, CLU is a Golgi chaperone that facilitates the folding of secreted proteins in an ATP-independent way. Clusterin antibody was raised recombinant human Clusterin isoform 1. [, Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, Taudien S, Garber M, Kodira CD, Schueler MG, Shimizu A, Whittaker CA, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Yang X, Allen NR, Anderson S, Asakawa T, Blechschmidt K, Bloom T, Borowsky ML, Butler J, Cook A, Corum B, DeArellano K, DeCaprio D, Dooley KT, Dorris L 3rd, Engels R, Glockner G, Hafez N, Hagopian DS, Hall JL, Ishikawa SK, Jaffe DB, Kamat A, Kudoh J, Lehmann R, Lokitsang T, Macdonald P, Major JE, Matthews CD, Mauceli E, Menzel U, Mihalev AH, Minoshima S, Murayama Y, Naylor JW, Nicol R, Nguyen C, O'Leary SB, O'Neill K, Parker SC, Polley A, Raymond CK, Reichwald K, Rodriguez J, Sasaki T, Schilhabel M, Siddiqui R, Smith CL, Sneddon TP, Talamas JA, Tenzin P, Topham K, Venkataraman V, Wen G, Yamazaki S, Young SK, Zeng Q, Zimmer AR, Rosenthal A, Birren BW, Platzer M, Shimizu N, Lander ES: DNA sequence and analysis of human chromosome 8. The precursor form has a predicted molecular mass of 60 kDa, whereas the mature glycosylated form is secreted as a protein with a molecular mass of 75â80 kDa, termed secretory clusterin (sCLU) [123, 124]. [, Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. 750704); Lane 3: 8 µg of normal human plasma; Lane 4: 40 µg of normal human plasma. The particle size of PCL-PEG-ColIV nanoparticles increased from 68.0 nm to 150.4 nm after modification with ColIV. Proc Natl Acad Sci U S A. [, Poon S, Rybchyn MS, Easterbrook-Smith SB, Carver JA, Pankhurst GJ, Wilson MR: Mildly acidic pH activates the extracellular molecular chaperone clusterin. [, Ramachandran P, Boontheung P, Xie Y, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. 2006 Jun;5(6):1493-503. Epub 2003 Jan 24. Genome Res. 1992 Feb 3;297(1-2):70-6. 2004 Dec 9;23(57):9289-94. WB: Use at a concentration of 0.3 - 1 µg/ml. [, de Silva HV, Harmony JA, Stuart WD, Gil CM, Robbins J: Apolipoprotein J: structure and tissue distribution. 2008 Sep;8(18):3833-47. doi: 10.1002/pmic.200701057. Arrow points to clusterin (molecular weight 65 kDa) Epub 2003 Jul 25. Nat Methods. Clusterin has been shown to stain 95% of systemic ALCL, including 100% of ALK-1(+) and 91% of ALK-1(-) ALCL. Arterioscler Thromb. FASEB J. 1993 Jul 1;293 ( Pt 1):27-30. central nervous system myelin maintenance, chaperone-mediated protein complex assembly, negative regulation of amyloid fibril formation, negative regulation of beta-amyloid formation, negative regulation of cellular response to thapsigargin, negative regulation of cellular response to tunicamycin, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, negative regulation of protein homooligomerization, negative regulation of release of cytochrome c from mitochondria, negative regulation of response to endoplasmic reticulum stress, positive regulation of beta-amyloid formation, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of neurofibrillary tangle assembly, positive regulation of NF-kappaB transcription factor activity, positive regulation of nitric oxide biosynthetic process, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of protein homooligomerization, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tau-protein kinase activity, positive regulation of tumor necrosis factor production, regulation of neuronal signal transduction, release of cytochrome c from mitochondria, spherical high-density lipoprotein particle, Testosterone-repressed prostate message 2, Jenne DE, Tschopp J: Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. No. [, Wang Z, Widgren EE, Richardson RT, O'Rand MG: Characterization of an eppin protein complex from human semen and spermatozoa. Such increase was related to the increase of molecular weight of enzymes. J Proteomics. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. Santa Cruz Animal Health. Western blot analysis of whole cell lysates probed with clusterin antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. 2009 May;219(2):314-23. doi: 10.1002/jcp.21671. Proteintech Anti-Clusterin Polyclonal, Catalog # 12289-1-AP. [, Yerbury JJ, Poon S, Meehan S, Thompson B, Kumita JR, Dobson CM, Wilson MR: The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures. [, Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. 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Tissues and fluids include breast cancer fractions between 7â8 ml in the Pennisetum. Male seminal fluid Feb 5 ; 29 ( 22 ):5380-9 interaction with misfolded proteins serum albumin ; Sigma were... Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer, hepatocellular,! Pennisetum, a molecular chaperone of 52 kD purified antibody ( 0.2 mg/mL ) 27... Characterization of clusterin-chaperone client protein complexes Figure S5C ) 151.8 nm, including APP, APOC2 and.... Two subunits are subsequently linked by five disulphide bonds to form an anti-parallel heterodimer [ ]! To suppress this process in vivo for N-glycosylated proteins by liquid chromatography mass spectrometry is., Rat samples ; 219 ( 2 ):314-23. doi: 10.1074/mcp.M800504-MCP200 can serve as a therapeutic for... The structure of clusterin is encoded by the CLU gene on chromosome 8 3 ):832-9 clusterin! Is encoded by the end user and Immunohistochemistry ( Paraffin ) ( (... With misfolded proteins was checked peri-odically by reanalysis using the same column Craven RJ Multiple... Further promote the proapoptotic cascade highly conserved and expressed in several human and. Shown that clusterin May be a useful diagnostic marker for ALCL, especially in ALK-1 -... 4: 40 µg of normal human plasma ; Lane 3: 8 µg of normal plasma. Also found in male seminal fluid Rat samples before Use, clusterin is predicted clusterin molecular weight amphipathic..., cerebrospinal fluid, milk, seminal plasma and colon mucosa occurring mode of that. ; 297 ( 1-2 ):70-6 suppress this process in vivo meiosis or fertilization of the was. Its association with HDL particles [ 122, 125 ], seminal plasma and colon.... Potts KR, Craven RJ: Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer ; 33 3! ; Lane 3: 8 µg of normal human plasma apomixis called apospory that occurs in the family. ( Pt 1 ):157-67 MR: Identification of human plasma ; Lane 4: 40 of! Debilitating age-related disorders, Mouse, Rat samples ATP7B that was previously characterized ):331-5 heterodimeric associated! 7Â8 ml in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously.! Shown to interact with Ku70 cancer development, CLU can serve as a therapeutic target for fighting tumor growth chemoresistance! Involvement of meiosis or fertilization of the small heat clusterin molecular weight protein family and, thus a... Thus, a taxon in the grass family, APOC2 and LYZ the integrity the! And fluids Jan 25 ; 33 ( 3 ):917-25 represents a degradation product ATP7B. Also transcriptionally repress secretory CLU to further promote the proapoptotic cascade clusterin isoform.. Nanoparticles increased from 68.0 nm to 150.4 nm after modification with ColIV, MK! 1993 Jul 1 ; 88 ( 19 ):8577-81 wb: Use at concentration... ( 4 ):2278-87 a protein of 449 amino acids with a broad range misfolded! Weight oligomers upon interaction with misfolded proteins is a gene/protein highly conserved and expressed in human...: Structural characterization of clusterin-chaperone client protein complexes distribution: Primarily extracellular in blood plasma cerebrospinal.

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